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3 . 2019

Analysis of amino acid composition of skim milk and buttermilk for the production of dairy drink when introducing whey protein hydrolysate

Abstract

The imbalance of macronutrient composition of people’s diets determines the relevance of the functional foods development - sources of protein and essential amino acids.

The aim of the work was to analyze the amino acid composition and determine the functional significance of the product based on skim milk and buttermilk byadding whey protein hydrolysate.

Material and methods. The control options were samples of skimmed milk, buttermilk and their mixtures in a 1:1 ratio. From 1 to 3% of the whey protein hydrolysate with a degree of hydrolysis of about 60% of peptide bonds were added to the same milk base samplesin the experimental options. The amino acid composition was analyzed by high performance liquid chromatography using a cation exchange analyzer and subsequent post-column derivatization with ninhydrin.

Results and discussion. The content of essential amino acids per 100 g of whey protein hydrolysate (WPH) is 50.6-168.0% from the adequate level of their daily intake, while there are only 3.0-22.3 and 2.6-19.9% in skimmed milk and buttermilk, respectively. Despite the practically equal protein content in skimmed milk and buttermilk 3.36±0.02 and 3.09±0.01%, respectively, the absolute content of all essential amino acids in buttermilk is lower than in skimmed milk. This can be explained by differences in the qualitative composition of proteins in these types of dairy raw materials.

Conclusion. The amino acid composition of the milk base, consisting of buttermilk and skimmed milk mixture in a 1:1 ratio, can be presented as «functional» product in relation to Val, He, Leu, Lis, Trp and Tyr, due to the addition of 3% WPH, because the product portion of 200 g provides 15% of adequate daily intake of these essential amino acids. There is the reason to include additional information: low-fat, low-calorie, contains only natural sugars and a source of protein for people whose protein requirement does not exceed 99 grams per day in the labeling of the developed product, in addition to its nutritional and energy value.

Keywords:whey protein hydrolysate, skimmed milk, buttermilk, amino acid composition, functional food, functional food ingredient

For citation: Novokshanova A.L., Topnikova Ye.V., Ababkova A.A. Analysis of amino acid composition of skim milk and buttermilk package for the production of dairy drink when introducing whey protein hydrolysate. Voprosy pitaniia [Problems of Nutrition]. 2019; 88 (3): 90-6. doi: 10.24411/0042-8833-2019-10034. (in Russian)

References

1. Bannikova A.V., Evdokimov I.A. Milk products enriched with whey proteins. Technological aspects of developing. Molochnaya promyshlennost’ [Dairy Industry]. 2015; (1): 64-6. (in Russian)

2. Bogdanova E.V., Melnikova E.I. Hydrolysates of whey proteins in the technology of products for specialized sports nutrition. Molochnaya promyshlennost’ [Dairy Industry]. 2018; (4): 45-7. (in Russian)

3. Koroleva O.V., Agarkova E.Yu., Botina S.G., Nikolaev I.V., Ponomareva N.V., Melnikova E.I., et al. Prospects of applying whey protein hydrolysates in the fermented milk products technology. Molochnaya promyshlennost’ [Dairy Industry]. 2013; (7): 66-8 (in Russian)

4. Martinchik A.N., Mayev I.V., Yanushevich O.O. General nutrition Research. Moscow: MEDpress-inform, 2005: 392 p.

5. Kodentsova V.M., Vrzhesinskaya O.A., Risnik D.V., Nikityuk D.B., Tutelyan V.A. Micronutrient status of population of the Russian Federation and possibility of its correction. State of the problem. Voprosy pitaniia [Problems of Nutrition]. 2017; 86 (4): 113-24. doi: 10.24411/0042-88 (in Russian)

6. Boyarskaya L.A., Vil’ms E.A., Turchaninov D.V., Bogdashin I.V., Erofeev Yu.V. Hygienic substantiation of application of functional dairy products in the prevention of macro- and micronutrient deficiency. Gigiena i sanitariya [Hygiene and Sanitation]. 2016; 11 (95): 1095-9. doi: 10.18821/0016-9900-2016-95-11-1095-1099

7. Institute of Medicine, Panel on Macronutrients, and Standing Committee on the Scientific Evaluation of Dietary Reference Intakes. Dietary reference intakes for energy, carbohydrate, fiber, fat, fatty acids, cholesterol, protein, and amino acids (Vol. 1). Natl Academy Pr., 2005.

8. Bandegan Arash. Indicator Amino Acid Derived Estimates of Dietary Protein Requirement in Exercise-Trained Individuals. 2016. Electronic Thesis and Dissertation Repository. 4055. https://ir.lib.uwo.ca/etd/4055

9. Cermak N.M., Res P.T., de Groot L., Saris W.H.M., van Loon L.J.C. Protein supplementation augments the adaptive response of skeletal muscle to resistance-type exercise training: a meta-analysis. Am J Clin Nutr. 2012; 96 (6): 1454-64.

10. Snijders T., Smeets J. S., van Vliet S., van Kranenburg J., Maase K., Kies A K., et al. Protein ingestion before sleep increases muscle mass and strength gains during prolonged resistance-type exercise training in healthy young men. J Nutr. 2015; 145: 1178-84.

11. Rafii M., Chapman K., Elango R., Campbell W.W., Ball R.O., Pencharz P.B., et al. Dietary protein requirement of men >65 years old determined by the indicator amino acid oxidation technique is higher than the current estimated average requirement. J Nutr. 2016; 146 (4): 681-7.

12. Sviridenko Yu.Ya., Magkonosov D.S., Abramov D.V., Ovchinnikova Ye.G. The technology of whey protein hydrolysates when applying a membrane technique. Part 1. Selection of enzyme preparation for hydrolysis in an enzymatic membrane reactor. Pishchevaya promyshlennost’ [Food Industry]. 2017; (7): 46-8. (in Russian)

13. Sviridenko Yu.Ya., Magkonosov D.S., Abramov D.V., Ovchinnikova Ye.G. The technology of whey protein hydrolysates when applying a membrane technique. Part 2. The technological optimization of whey protein hydrolysates in an enzymatic membrane reactor. Pishchevaya promyshlennost’ [Food Industry]. 2017; (8): 40-3. (in Russian)

14. Abramov D.V., Sviridenko Yu.Ya., Myagkonosov D.S., Ovchinnikova E.G., Kangin M.P., Kokareva N.V. Development of whey proteins enzymatic hydrolysates - technology, properties and application. http://www.dairynews.ru/processing/razrabotka-fermentativnykh-gidrolizatov-syvorotoch.html. (dare of access January 20, 2018)

15. Ababkova A.A., Abramov D.V., Novokshanova A.L. A drink with hydrolyzed whey milk proteins. Molochnaya promyshlennost’ [Dairy Industry]. 2016; (12): 58-60. (in Russian)

16. Novokshanova A.L., Ababkova A.A., Ivanova S.V. Inoculation dose determination of whey proteins hydrolysate into fermented milk product by means of organoleptic evaluation. Molochnokhozyaistvenniy vestnik[Dairy Bulletin]. 2015; 1 (17): 79-86. (in Russian)

17. Vyshemirskiy F.A., Azhgikhina N.N. Buttermilk: the minimum of calories is the maximum of biological value.Molochnaya promyshlennost’ [Dairy Industry]. 2011; (9): 54-6. (in Russian)

18. Food Chemistry. In: Sh. Damodaran, K.L. Parkin, O.R. Fennem (eds). trans. from English. Saint Petersburg: Professiya, 2012: 1040 p.

19. Tepel A. Chemistry and physics of milk. Saint Petersburg: Professiya, 2012: 832 p.

20. Ababkova A.A., Neronova E.Yu., Novokshanova A.L. Studying of rheological characteristics of fermented curds enriched by the hydrolysis of whey proteins. Molochnokhozyaistvenniy vestnik [Dairy Bulletin]. 2016; 3 (23): 37-45. (in Russian)

21. MR 2.3.1.1915-04 Methodical recommendations. Nutrition Recommended levels of consumption of food and biologically active substances.

22. Norms of physiological needs in energy and nutrients for various groups of the population of the Russian Federation: methodological recommendations. MR 2.3.1.2432-08. Moscow: Minzdrav RF, 2008: 41 p. (in Russian)

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CHIEF EDITOR
CHIEF EDITOR
Viktor A. Tutelyan
Full Member of the Russian Academy of Sciences, Doctor of Medical Sciences, Professor, Scientific Director of the Federal Research Centre of Nutrition, Biotechnology and Food Safety (Moscow, Russia)

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