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6 . 2018

Determination of phthalates in juice product by high-performance liquid chromatography/mass spectrometry

Abstract



The paper presents the research results obtained after determination of the persistent organic pollutants of the environment - phthalates in domestic juice products with the use of high-performance liquid chromatography/ mass spectrometry (HPLC/MS/MS) with application of solid-phase extraction as a method of sample preparation. Selective determination of phthalates in fruit and vegetable juices by HPLC/MS/MS method has been provided through parent and child ions’ monitoring that are formed during ionization of the molecules of the studied compounds in the electrostatic spray of isomers on a reversed-phase column. The analysis of the samples of juice product revealed the presence of 11 phthalates out of 13 studied in concentration range from 0.4 up to 59.26 mg/dm3. The maximum amount of phthalates was found in juices with pulp 31.9-59.26 mg/dm3 (tetrahedral package), the minimum content of phthalates 0.4 mg/dm3 was detected in flavoured beverages (polyethylene terephthalate packing) and 1 mg/dm3 in the fruit-and-vegetable nectar (glass packing). The most samples contained from 3 to 8 phthalates with total content of 0.4-5.82 mg/dm3. The most prevailing pollutants of the analyzed samples were di-n-octylphthalate found in 100% of samples, dinonyl phthalate and di (2-ethylhexyl) phthalate, presented in 70-80% of samples. The maximum pollution density of the juice samples was noted for di-n-octyl phthalate, di (2-ethylhexyl) phthalate, diisononyl phthalate and diisobutyl phthalate.

Keywords:high-performance liquid chromatography, mass spectrometric analysis, juice products, phthalates

Voprosy pitaniia [Problems of Nutrition]. 2018; 87 (6): 117-24. doi: 10.24411/0042-8833-2018-10073.

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CHIEF EDITOR
CHIEF EDITOR
Viktor A. Tutelyan
Full Member of the Russian Academy of Sciences, Doctor of Medical Sciences, Professor, Scientific Director of the Federal Research Centre of Nutrition, Biotechnology and Food Safety (Moscow, Russia)

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