To the content
6 . 2018

Biodegradation of proteins of grain raw materials for the production of new bakery products

Abstract



The effect of enzyme systems on the degree of protein destruction of grain crops to obtain new types of bakery products has been studied. Protein and amino acid composition of triticale grain crop in comparison with wheat and rye one has been studied. The high biological value of triticale proteins containing 38.75% of essential amino acids while in wheat - 34.93% has been shown. The influence of different enzyme systems (ES) with proteolytic action on the efficiency of catalytic modification of triticale proteins has been investigated. It was found that the highest activity was shown by the enzymatic system ES-1, synthesized by the mycelial fungus Aspergillus oryzae, as a result of which at a concentration of 5 u/g, the level of accumulation of amine nitrogen in triticale enzymatic hydrolysates was 125 mg%; the degree of hydrolysis of proteins was 90%. Enzyme preparations of bacterial origin, as well as alkalase and papain had a lower ability to hydrolyze triticale proteins. The fractional composition of modified proteins obtained by ES-1 showed a decrease in their molecular weight (to 35 kDa). Analysis of amino acid composition in grain enzymatic hydrolysates showed that as a result of exposure to FS-1, about 50% of the total number of amino acids passed into the free state, of which 38.8 to 43.6% were essential amino acids. The recipes of breads, containing composition of wheat flour and fermentolizates of the whole-grain triticale flour in the ratio 1:1 have been tested. The amino acid composition of the bread showed that the test samples contained 6.2 fold more free amino acids than the control. The use of fermented triticale in the recipes of bread allowed to increase the content of essential amino acids such as methionine, valine, isoleucine, leucine, phenylalanine, threonine, tryptophan and lysine in 2.0-5.0 times. It was shown that the developed technology allowed baking bread containing peptides with reduced molecular weight and free amino acids, which by its organoleptic and physic-chemical parameters corresponded to classic bakery products.

Keywords:enzymatic hydrolysis, cereal proteins, proteases, amino acids, bakery products, hypoallergenic products

Voprosy pitaniia [Problems of Nutrition]. 2018; 87 (6): 67-75. doi: 10.24411/0042-8833-2018-10068.

All articles in our journal are distributed under the Creative Commons Attribution 4.0 International License (CC BY 4.0 license)

SCImago Journal & Country Rank
Scopus CiteScore
CHIEF EDITOR
CHIEF EDITOR
Viktor A. Tutelyan
Full Member of the Russian Academy of Sciences, Doctor of Medical Sciences, Professor, Scientific Director of the Federal Research Centre of Nutrition, Biotechnology and Food Safety (Moscow, Russia)

Journals of «GEOTAR-Media»