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6 . 2015

Effect of minor bioactive food substances – rutin and hesperidin in their separate and combined alimentary arrives on the immune system of rats and the activity of nuclear factor NF-kB liver cells

Abstract

The effect of rutin and hesperidin in their separate and combined admission to the immune system and the activity of nuclear factor NF-kB of rat liver cells has been investigated. Wistar male rats with an initial body weight of 224–225 g were divided into 4 groups of 6 rats in each. The rats of the 1st group (control) received a complete semi-synthetic diet, rats in group 2 – the same diet supplemented with rutine (400 mg/kg b.w.); the rats of group 3 – with the addition of hesperidin (400 mg/kg bw); group 4 – with the addition of rutin and hesperidin (400 mg/kg b.w. each) for 14 days. Animals received feed in free access mode in an amount of 25–30 g per rat per day, that corresponded to 15 g of dry formula. Animals received water also in free access. It has been found that rutin and hesperidin, included in the diet of rats both alone (groups 2 and 3) and together (group 4), have immunomodulatory impact which is a reduce of lymphocyte relative content [1st gr. – 70.55±1.58%, 2nd gr. – 63.62±2.85%, 3rd gr. – 62.03±3.16% (p1–3<0.05), 4th gr. – 65.75±1.08% (p1–4<0.05)] and an increase of percentage of neutrophil leukocytes [1st gr – 19.98±0.97%, 2nd gr. – 25.35±3.14%, 3rd gr. – 28.27±3.30% (p1–3<0.05), 4th gr. – 24.15±1.52% (p1–4<0.05)] and NK-cells in the peripheral blood [1st gr. – 3.29±0.45%, 2nd gr. – 6.91±0.70% (p1–2<0.05), 3rd gr. – 5.88±0.79% (p1–3<0.05), 4th gr. – 4.64±0.32% (p1–4<0.05)], that can be considered as a shift in the direction of innate immunity factors.

In addition, the combined effect of high doses of rutin and hesperidin led to a change in erythrocyte parameters: an increase in the average volume of red blood cells [1st gr. – 56.00±1.06 fl, 2nd gr. – 56.67±0.42 fl, 3rd gr. – 58.50± 0.99 fl, 4th gr. – 59.50±0.99 fl (p1–4<0.05)], and the average content of hemoglobin [1st gr. – 18.97±0.45 pg, 2nd gr. – 19.10±0.19 pg, 3rd gr. – 19.73± 0.32 pg, 4th gr. – 20.08±0.33 pg (p1–4=0.07)], as well as increase in the level of TGF-β1 in peripheral blood [1st gr. – 15.55±2.13 ng/ml, 2nd gr. – 14.81± 2.36 ng/ml, 3rd gr. – 17.02±2.53 ng/ml, 4th gr. – 22.14±2.29 ng/ml (p1–4<0.05)] and the expression of nuclear factor NF-kB in the liver cells [1st gr. – 16.10± 0.60 ng/ml; 2nd gr. – 15.14±2.28 ng/ml; 3rd gr. – 15.85±2.09 ng/ml; 4th gr. – 20.49±1.68 ng/ml (p1–4<0.05)].

Keywords:flavonoids, rutin, hesperidin, immunity, hematology, lymphocyte subpopulations, cytokines, nuclear factor NF-kB

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CHIEF EDITOR
CHIEF EDITOR
Viktor A. Tutelyan
Full Member of the Russian Academy of Sciences, Doctor of Medical Sciences, Professor, Scientific Director of the Federal Research Centre of Nutrition, Biotechnology and Food Safety (Moscow, Russia)

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