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1 . 2014

The effect of diet ratio of polyunsaturated fatty acids of ω-3 and ω-6 families on activity of aminotransferases and γ-glutamyltransferase in rat blood serum

AbstractThe effect of diet fat compositions with various ratio of ω-3 and ω-6 polyunsaturated fatty acids (PUFAs) on alanine aminotransferase (ALT), aspartate aminotransferase (AST) and γ-glutamyltransferase (GGT) activities in blood serum of 45 white mongrel rats weighing 90–110 g (9 animals in group) has been investigated. Fat components in the semi-synthetic diet, compiled on the basis of AIN-93 diet, and sources of ω-6 and ω-3 PUFA were presented by sunflower oil, soybean oil and fish oil. It has been shown that four-week inclusion of linoleic acid (LA) and α-linolenic acid (α-LNA) in a ratio of 7:1 into the diet (soybean oil) as well as use of only ω-6 PUFA (sunflower oil) has lead to an increase in the activity of ALT and GGT in rat blood serum compared to control animals treated with the complex of linolenic, eicosapentaenoic (EPA) and docosahexaenoic (DHA) acid through the mixture of sunflower oil and fish oil (9:1) with the ratio of ω-6 and ω-3 PUFA 7:1. Along with this, the AST:ALT ratio (de Ritis ratio) was lower (p<0,05) as compared with the control group of rat, amounting respectively 0,92±0,08 and 0,79±0,12 vs 1,26±0,10. The use of high doses of ω-3 fatty acids (600 mg EPA and 400 mg DHA per kg of animal weight per day coming through fish oil) did not affect the activity of ALT and GGT, but increased AST serum activity (0,47±0,04 micromoles/min per mg protein) and the de Ritis ratio (2,53±0,23). The diet deprived with fat increased enzyme activity of ALT, AST and GGT in rat blood serum.

Keywords:alanine aminotransferase, aspartate aminotransferase, γ-glutamyltransferase, polyunsaturated fatty acids

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CHIEF EDITOR
CHIEF EDITOR
Viktor A. Tutelyan
Full Member of the Russian Academy of Sciences, Doctor of Medical Sciences, Professor, Scientific Director of the Federal Research Centre of Nutrition, Biotechnology and Food Safety (Moscow, Russia)

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